Seventeen Minutes of Science: The Establishment and Growth of
Zebrafish as a Model System.
Tune in weekly to our virtual series “Seventeen Minutes of Science” every Tuesday at 11am PST / 2pm ET where we go live on …
Fueling Discovery
Gene By Gene
Deliver answers more effectively by using alternative, non-mammalian model organisms in early biological
and biomedical research.
Who We Are
About InVivo Biosystems
InVivo Biosystems provides essential services to help pharmaceutical,
nutracecal, biotechnology companies and academic research institutions around the globe accelerate their research and drug development efforts.
An expert in CRISPR genome editing, InVivo Biosystems creates custom genome edited C. elegans and zebrafish models to enable aging, and disease studies. InVivo Biosystems also develops and manufactures genotyping and phenotyping products, including instruments, reagents and consumables to allow researchers to explore and discover new phenotypes.
Our Service
What We Do
The InVivo Biosystems Technology provides the deep insights you need to make a go/no-go decision. Our data analysis and focused team of experts will also help you to understand the results and identify alternate routes and hypotheses you may not have seen previously.
CRISPR Genome Editing
InVivo Biosystems is a licensed CRISPR/Cas9 gene editing solutions provider.
Lifespan + Healthspan
We offer a streamlined Longevity Platform to determine whether a compound can extend lifespan.
Toxicity Testing
Toxicity studies using mammalian models are expensive and time- consuming1, 2.
Analytical Services
Our early in-vivo analytical testing uses relevant and time-efficient whole animal models to help you shorten your project timeline.
How We Help
What Would You Like To Do?
Modify
Genome-edited C. elegans or zebrafish built to your specification.
Prepare
Start critical first steps in your experiment with worm-prep reagents & tools.
Measure
Visualize, measure C. elegans behavior and collect phenotypic output.
Our Guides
Point Mutation Publications
Single Amino Acid Changes in the Ryanodine Receptor in the Human Population Have Effects In Vivo on Caenorhabditis elegans Neuro-Muscular Function
Repurposing the aldose reductase inhibitor and diabetic neuropathy drug epalrestat for the congenital disorder of glycosylation PMM2-CDG
Regulation of the sperm- to-oocyte transition in Caenorhabditis briggsaehermaphrodites by the Cbr-met-2 and Cbr-fem-3 genes
Let’s Connect With Us
Ready to connect with us to learn more about working with our company or our technology?
Flow Chart
The InVivo Biosystems Workflow
InVivo Biosystems provides a streamlined process for zebrafish gene editing services.
Project Design
Selection of best guide RNAs surrounding the locus to be edited.
01
sgRNA Testing
In vivo cutting assayed by PCR/ sequencing to determine the most efficient sgRNA.
02
Donor Homology Design
Repair templates are designed around high quality sgRNAs.
03
Germline Verification
Verification of germline editing
06
Clutch Verification
Evaluation of edit by PCR and/or sequencing of 10% of injected embryos.
05
Injection
Skilled injectors for rigorous in vivo testing of editing reagents.
04
Our Products
The Zebrafish Embryonic Genotyper (ZEG)
Rapid Automated DNA Extraction for Live Zebrafish Embryos
- LIVE genotyping of embryos that can be raised to adulthood
- RAPID cell extraction (96 embryos in an 1 hour)
- EASY-TO-USE Minimal training required to get up and running
- UNIQUE capabilities make out-of-reach experiments possible
- SAVE time and facility fees
Comparison
Comparison of Zebrafish Genome Editing Methods
Tol2 Transposase
CRISPR/Cas9
Morpholinos (MOs)
Tol2 Transposase
Tol2 Transposase
Most commonly used in zebrafish labs to make fast and rapid genome edits
by random insertion of reporter cassettes (e.g. GFP, overexpression of mutant proteins). However, this method is not locus specific. It does not always recapitulate native expression patterns of gene and does not produce disruption in WT sequence.
InVivo Biosystems does not used the Tol2 method to produce mutants.
CRISPR/Cas9
Tol2 Transposase
Most commonly used in zebrafish labs to make fast and rapid genome edits
by random insertion of reporter cassettes (e.g. GFP, overexpression of mutant proteins). However, this method is not locus specific. It does not always recapitulate native expression patterns of gene and does not produce disruption in WT sequence.
InVivo Biosystems does not used the Tol2 method to produce mutants.
Morpholinos (MOs)
Tol2 Transposase
Most commonly used in zebrafish labs to make fast and rapid genome edits
by random insertion of reporter cassettes (e.g. GFP, overexpression of mutant proteins). However, this method is not locus specific. It does not always recapitulate native expression patterns of gene and does not produce disruption in WT sequence.
InVivo Biosystems does not used the Tol2 method to produce mutants.
Offers Details
Injection Mix and sgRNA Validation Offerings
Standard Injection Mix
Standard Injection Mix + Screening Primers
Validated sgRNA Injection Mix
Complete Validated Injection Mix
Standard Injection Mix
Design
Injection Mix
Screening Reagents
In vivo sgRNA Testing
In vivo Editing Assesment
Pricing*
$995
Timeline
4 weeks
Standard Injection Mix + Screening Primers
Design
Injection Mix
Screening Reagents
In vivo sgRNA Testing
In vivo Editing Assesment
Pricing*
$1,995
Timeline
6 weeks
Validated sgRNA Injection Mix
Design
Injection Mix
Screening Reagents
In vivo sgRNA Testing
In vivo Editing Assesment
Pricing*
$3,995
Timeline
8-10 weeks
Complete Validated Injection Mix
Design
Injection Mix
Screening Reagents
In vivo sgRNA Testing
In vivo Editing Assesment
Pricing*
$5,995
Timeline
12+ weeks
Standard Injection Mix
Standard Injection Mix + Screening Primers
Validated sgRNA Injection Mix
Complete Validated Injection Mix
Design
Injection Mix
Screening Reagents
In vivo sgRNA Testing
In vivo Editing Assesment
Pricing*
$995
$1,995
$3,995
$5,995
Timeline
4 weeks
6 weeks
8-10 weeks
12+ weeks
Complete Validated Injection Mix
Choose this option if you want a CRISPR knockin with completely validated reagents to use in your lab and to find your gene edited line. This is the best Custom Injection Mix option for the novice zebrafish line builder.
OUR VALIDATED sgRNA INJECTION MIX PLUS:
- Full in vivo evaluation of somatic editing efficiency.
- Confirmation of integration capacity of your edit into the zebrafish genome
Full in vivo evaluation of somatic editing efficiency.
Confirmation of integration capacity of your edit into the zebrafish genome
Countaing The Stats
key Features
Single Amino Acid Changes in the Ryanodine Receptor in the Human Population Have Effects In Vivo on Caenorhabditis elegans Neuro-Muscular Function Single Amino Acid Changes in the Ryanodine Receptor in the Human Population Have Effects In Vivo on Caenorhabditis elegans Neuro-Muscular Function
1,325,317
over 1 million emails were sent
10,000
zebrafish were reared
327
inquiries about New projects
107,045
100,000 zebrafish embroys were injected & 7,045 worms were injected
65,410
unique visits to our website
FAQs
Frequently Asked Question
Dr. Schrader begins by making incisions within the nasal passages
to access the internal bones and cartilage that support the nose.
Depending on the desired result, Dr. Schrader may remove some
bone and cartilage or add tissue from another area of the body or
with a synthetic filler.
Dr. Schrader begins by making incisions within the nasal passages
to access the internal bones and cartilage that support the nose.
Depending on the desired result, Dr. Schrader may remove some
bone and cartilage or add tissue from another area of the body or
with a synthetic filler.
Dr. Schrader begins by making incisions within the nasal passages
to access the internal bones and cartilage that support the nose.
Depending on the desired result, Dr. Schrader may remove some
bone and cartilage or add tissue from another area of the body or
with a synthetic filler.
Dr. Schrader begins by making incisions within the nasal passages
to access the internal bones and cartilage that support the nose.
Depending on the desired result, Dr. Schrader may remove some
bone and cartilage or add tissue from another area of the body or
with a synthetic filler.
Dr. Schrader begins by making incisions within the nasal passages
to access the internal bones and cartilage that support the nose.
Depending on the desired result, Dr. Schrader may remove some
bone and cartilage or add tissue from another area of the body or
with a synthetic filler.
Dr. Schrader begins by making incisions within the nasal passages
to access the internal bones and cartilage that support the nose.
Depending on the desired result, Dr. Schrader may remove some
bone and cartilage or add tissue from another area of the body or
with a synthetic filler.
Dr. Schrader begins by making incisions within the nasal passages
to access the internal bones and cartilage that support the nose.
Depending on the desired result, Dr. Schrader may remove some
bone and cartilage or add tissue from another area of the body or
with a synthetic filler.
Dr. Schrader begins by making incisions within the nasal passages
to access the internal bones and cartilage that support the nose.
Depending on the desired result, Dr. Schrader may remove some
bone and cartilage or add tissue from another area of the body or
with a synthetic filler.
Resourses
Latest Blog & News
Seventeen Minutes of Science: The Establishment and Growth of
Seventeen Minutes of Science: The Establishment and Growth of
Zebrafish as a Model System.
Tune in weekly to our virtual series “Seventeen Minutes of Science” every Tuesday at 11am PST / 2pm ET where we go live on …
Seventeen Minutes of Science: The Establishment and Growth of
Zebrafish as a Model System.
Tune in weekly to our virtual series “Seventeen Minutes of Science” every Tuesday at 11am PST / 2pm ET where we go live on …
Start A Project
Interested In StartingYour Zebrafish Knock-In Tagging Project?
InVivo Biosystems provides a streamlined process for zebrafish gene editing services.
Submit your inquiry below & we will get back to you soon.
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An expert in CRISPR genome editing, InVivo Biosystems creates custom genome-edited C. elegans and zebrafish models to enable aging, developmental and disease studies. Our unique living platforms and technologies bridge the gap between cells and mice, providing faster.
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